| Assay Method Information | |
| | Amidolytic Activity Assay (Trypsin) |
| Description: | Determination of amidolytic activity was performed as described previously [Okada et al., Chem. Pharm. Bull., 36:1289-1297]. Detailed description of the method is given below. To 0.2 cm3 of examined compound (1−11; 0.15 M NaCl as control), buffer, and 0.1 cm^3 of enzyme solution was added: borane buffer−0.5 cm^3 (pH = 7.5); enzyme: trypsin (0.4 unit/cm^3), synthetic substrate: Bzl-l-Arg-pNA HCl(0.2 cm^3, 8 mM/dm^3). |
| Affinity data for this assay | |
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