BindingDB PrimarySearch

Found 20 hits Enz. Inhib. hit(s) with Target = 'DNA topoisomerase 2-alpha' and Ligand = 'BDBM50127140'
Target/Host (Institution)	Ligand	Target/Host Links	Ligand Links	Trg + Lig Links	Ki nM	ΔG° kJ/mole	IC50 nM	Kd nM	EC50/IC50 nM	k_off s^-1	k_on M^-1s^-1	pH	Temp °C
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	2.18E+4	n/a	n/a	n/a	n/a	n/a	n/a
Sichuan University Curated by ChEMBL					Assay Description Inhibition of topoisomerase 2				Bioorg Med Chem Lett 22: 3122-5 (2012) Article DOI: 10.1016/j.bmcl.2012.03.061 BindingDB Entry DOI: 10.7270/Q2SF2X5D
Sichuan University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	2.82E+4	n/a	n/a	n/a	n/a	n/a	n/a
Ewha Womans University Curated by ChEMBL					Assay Description Inhibition of human DNA topoisomerase-2alpha assessed as relaxation of supercoiled pBR322 DNA after 30 mins by agarose gel electrophoresis				Eur J Med Chem 69: 139-45 (2013) Article DOI: 10.1016/j.ejmech.2013.07.048 BindingDB Entry DOI: 10.7270/Q2JS9TCS
Ewha Womans University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	3.00E+4	n/a	n/a	n/a	n/a	7.8	37
Medical University					Assay Description Supercoiled pHOT1 DNA (0.5 μg) was incubated with four units of human topoisomerase I in relaxation buffer (10mM Tris-HCl (pH 7.8), 1mM EDTA, 0.15...				J Enzyme Inhib Med Chem 29: 243-8 (2014) Article DOI: 10.3109/14756366.2013.768987 BindingDB Entry DOI: 10.7270/Q2416W07
Medical University					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	3.38E+4	n/a	n/a	n/a	n/a	n/a	n/a
National Institute of Chemistry Curated by ChEMBL					Assay Description Inhibition of human DNA topoisomerase 2alpha using plasmid pNO1 substrate incubated at 37 degC for 30 mins by fluorimetry				Bioorg Med Chem Lett 24: 5762-8 (2014) Article DOI: 10.1016/j.bmcl.2014.10.042 BindingDB Entry DOI: 10.7270/Q24Q7WMK
National Institute of Chemistry Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	3.38E+4	n/a	n/a	n/a	n/a	n/a	n/a
Shandong University Curated by ChEMBL					Assay Description Inhibition of recombinant human topoisomerase 2alpha using supercoiled pNO1 plasmid as substrate after 30 mins by SYBR-GOLD staining based fluorimetr...				J Med Chem 61: 8947-8980 (2018) Article DOI: 10.1021/acs.jmedchem.7b01202 BindingDB Entry DOI: 10.7270/Q2H998M5
Shandong University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	4.75E+4	n/a	n/a	n/a	n/a	n/a	n/a
Istituto Italiano di Tecnologia Curated by ChEMBL					Assay Description Inhibition of human topo2alpha incubated for 30 mins by SYBR safe DNA stain based decatenation assay				J Med Chem 63: 3508-3521 (2020) Article DOI: 10.1021/acs.jmedchem.9b01760 BindingDB Entry DOI: 10.7270/Q2251NHX
Istituto Italiano di Tecnologia Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB PubMed	n/a	n/a	5.00E+4	n/a	n/a	n/a	n/a	n/a	n/a
Osaka University of Pharmaceutical Sciences Curated by ChEMBL					Assay Description Inhibition of human 1 unit topoisomerase 2alpha catalytic activity assessed as relaxation of 198 ng supercoiled pBR322 DNA by agarose gel electrophor...				J Nat Prod 64: 1545-7 (2001) BindingDB Entry DOI: 10.7270/Q2X92C63
					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	5.02E+4	n/a	n/a	n/a	n/a	n/a	n/a
Kindai University Curated by ChEMBL					Assay Description Inhibition of human placenta topoisomerase 2alpha assessed as reduction in pBR322 supercoiled DNA relaxation after 30 mins by ethidium bromide staini...				Bioorg Med Chem 26: 1920-1928 (2018) Article DOI: 10.1016/j.bmc.2018.02.042 BindingDB Entry DOI: 10.7270/Q2RR21WV
Kindai University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	5.60E+4	n/a	n/a	n/a	n/a	n/a	n/a
University of Manitoba Curated by ChEMBL					Assay Description Inhibition of human DNA topoisomerase 2alpha-mediated decatenation of kinetoplast plasmid DNA by fluorescent plate reader				Bioorg Med Chem 18: 830-8 (2010) Article DOI: 10.1016/j.bmc.2009.11.048 BindingDB Entry DOI: 10.7270/Q2KH0P8X
University of Manitoba Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	5.60E+4	n/a	n/a	n/a	n/a	n/a	n/a
Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Curated by ChEMBL					Assay Description Inhibition of recombinant human DNA topoisomerase-2alpha assessed as decrease in relaxation of supercoiled pBR322 DNA after 30 mins by ethidium bromi...				Bioorg Med Chem 25: 4133-4144 (2017) Article DOI: 10.1016/j.bmc.2017.06.001 BindingDB Entry DOI: 10.7270/Q2D79DWS
					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	5.95E+4	n/a	n/a	n/a	n/a	7.9	n/a
Jamia Millia Islamia (A Central University)					Assay Description Relaxation of negatively supercoiled plasmid DNA by human topoisomerase IIα was assayed in 20 �L of reaction buffer (10 mM Tris-HCl, pH 7.9, 150...				Bioorg Chem 69: 77-90 (2016) Article DOI: 10.1016/j.bioorg.2016.10.001 BindingDB Entry DOI: 10.7270/Q21V5CS9
Jamia Millia Islamia (A Central University)					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	6.03E+4	n/a	n/a	n/a	n/a	n/a	n/a
Sun Yat-sen University Curated by ChEMBL					Assay Description Inhibition of human topoisomerase 2 alpha-mediated relaxation of supercoiled pBR322 DNA after 30 mins by agarose gel electrophoresis				Eur J Med Chem 46: 3339-47 (2011) Article DOI: 10.1016/j.ejmech.2011.04.059 BindingDB Entry DOI: 10.7270/Q2513ZJD
Sun Yat-sen University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	6.83E+4	n/a	n/a	n/a	n/a	n/a	n/a
TBA					Assay Description Inhibition of human DNA topoisomerase 2alpha assessed as suppression of decatenation using catenated kinetoplast DNA as substrate measured after 30 m...				Citation and Details Article DOI: 10.1016/j.ejmech.2020.112504 BindingDB Entry DOI: 10.7270/Q2VT1WRN
TBA					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	7.10E+4	n/a	n/a	n/a	n/a	n/a	n/a
TBA					Assay Description Inhibition of human topoisomerase 2alpha using supercoiled pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence based microplate reade...				Citation and Details Article DOI: 10.1039/c9md00597h BindingDB Entry DOI: 10.7270/Q29S1VQG
TBA					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB PubMed	n/a	n/a	7.57E+4	n/a	n/a	n/a	n/a	n/a	n/a
Osaka University of Pharmaceutical Sciences Curated by ChEMBL					Assay Description Inhibition of human DNA topoisomerase 2alpha				J Nat Prod 65: 1657-9 (2002) BindingDB Entry DOI: 10.7270/Q25D8RMH
					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	7.84E+4	n/a	n/a	n/a	n/a	n/a	n/a
National Institute of Pharmaceutical Education and Research Curated by ChEMBL					Assay Description Inhibition of human topoisomerase-2alpha using kDNA as substrate assessed as inhibition of ATP-dependent kDNA decatenation after 30 mins by agarose g...				Bioorg Med Chem 21: 5782-93 (2013) Article DOI: 10.1016/j.bmc.2013.07.016 BindingDB Entry DOI: 10.7270/Q2154M0M
					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	7.84E+4	n/a	n/a	n/a	n/a	n/a	n/a
Central University of Punjab Curated by ChEMBL					Assay Description Inhibition of human topoisomerase-2 alpha assessed as reduction in enzyme-mediated kinetoplast DNA decatenation incubated for 30 mins by ethidium bro...				Bioorg Med Chem 23: 5654-61 (2015) Article DOI: 10.1016/j.bmc.2015.07.020 BindingDB Entry DOI: 10.7270/Q2XK8HBK
Central University of Punjab Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	9.29E+4	n/a	n/a	n/a	n/a	n/a	n/a
Ewha Womans University Curated by ChEMBL					Assay Description Inhibition of human recombinant topoisomerase 2 by decatenation assay				Bioorg Med Chem 16: 4545-50 (2008) Article DOI: 10.1016/j.bmc.2008.02.049 BindingDB Entry DOI: 10.7270/Q20Z745T
Ewha Womans University Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	1.20E+5	n/a	n/a	n/a	n/a	n/a	n/a
TBA					Assay Description Inhibition of recombinant human topoisomerase 2alpha using supercoiled pBR322 DNA as substrate incubated for 60 mins by ethidium bromide dye based ag...				Citation and Details Article DOI: 10.1021/acs.jmedchem.0c00774 BindingDB Entry DOI: 10.7270/Q26H4N2X
TBA					More data for this Ligand-Target Pair				3D Structure (crystal)
DNA topoisomerase 2-alpha (Homo sapiens (Human))	BDBM50127140 ((-)-etoposide \| (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-d...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia GoogleScholar AffyNet	Purchase CHEMBL DrugBank MCE KEGG MMDB PC cid PC sid PDB UniChem Patents Similars	PDB Article PubMed	n/a	n/a	1.20E+5	n/a	n/a	n/a	n/a	n/a	n/a
Istituto Italiano di Tecnologia Curated by ChEMBL					Assay Description Inhibition of human topoisomerase 2alpha expressed in baculovirus-infected insect cells assessed as reduction in pBR322 supercoiled DNA relaxation af...				J Med Chem 61: 1375-1379 (2018) Article DOI: 10.1021/acs.jmedchem.7b01388 BindingDB Entry DOI: 10.7270/Q2B56N5T
Istituto Italiano di Tecnologia Curated by ChEMBL					More data for this Ligand-Target Pair				3D Structure (crystal)