Reaction Details |
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Target | Dimer of Gag-Pol polyprotein [482-580,I502K,I528M,T553A,V564F] |
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Ligand | BDBM519 |
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Substrate/Competitor | HIV Protease Chromogenic Peptide Substrate |
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Meas. Tech. | Protease Inhibition Assay |
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Ki | 0.43±n/a nM |
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Citation | Clemente, JC; Coman, RM; Thiaville, MM; Janka, LK; Jeung, JA; Nukoolkarn, S; Govindasamy, L; Agbandje-McKenna, M; McKenna, R; Leelamanit, W; Goodenow, MM; Dunn, BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry45:5468-77 (2006) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Dimer of Gag-Pol polyprotein [482-580,I502K,I528M,T553A,V564F] |
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Name: | Dimer of Gag-Pol polyprotein [482-580,I502K,I528M,T553A,V564F] |
Synonyms: | HIV-1 Protease AE Mutant (V82F) | HIV-1 Protease Mutant (V3I, I13V, E35D, M36I, S37N, R41K, H69K, V82F, L89M) | Pretherapy isolated AE protease mutant (V82F) |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21. |
Components: | This complex has 2 components. |
Component 1 |
Name: | Gag-Pol polyprotein [482-580,I502K,I528M,T553A,V564F] |
Synonyms: | HIV-1 Protease AE Mutant (V82F) Chain A | HIV-1 Protease AE Mutant (V82F) Chain B | POL_HV1U4 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10805.72 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P24740[482-580,I502K,I528M,T553A,V564F] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTVKIGGQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYD
QILIEICGKKAIGTVLVGPTPFNIIGRNMLTQIGCTLNF
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Component 2 |
Name: | Gag-Pol polyprotein [482-580,I502K,I528M,T553A,V564F] |
Synonyms: | HIV-1 Protease AE Mutant (V82F) Chain A | HIV-1 Protease AE Mutant (V82F) Chain B | POL_HV1U4 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10805.72 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P24740[482-580,I502K,I528M,T553A,V564F] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTVKIGGQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYD
QILIEICGKKAIGTVLVGPTPFNIIGRNMLTQIGCTLNF
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BDBM519 |
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HIV Protease Chromogenic Peptide Substrate |
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Name: | HIV Protease Chromogenic Peptide Substrate |
Synonyms: | n/a |
Type: | Peptide |
Mol. Mass.: | 1420.11 |
Organism: | n/a |
Description: | n/a |
Residue: | 13 |
Sequence: | |