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TargetBotulinum neurotoxin type A2 [1-425]
LigandBDBM23297
Substrate/CompetitorSNAP-25 substrate peptide
Meas. Tech.BoNT/A LC Metalloprotease Activity Assay
pH7.3±n/a
Temperature310.15±n/a K
IC50 17000±1700 nM
Citation Solaja, BA; Opsenica, D; Smith, KS; Milhous, WK; Terzić, N; Opsenica, I; Burnett, JC; Nuss, J; Gussio, R; Bavari, S Novel 4-aminoquinolines active against chloroquine-resistant and sensitive P. falciparum strains that also inhibit botulinum serotype A. J Med Chem51:4388-91 (2008) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Botulinum neurotoxin type A2 [1-425]
Name:Botulinum neurotoxin type A2 [1-425]
Synonyms:BXA2_CLOBJ | BoNT/A LC | Botulinum Neurotoxin Type A | Botulinum neurotoxin A light chain | atx | bna | bonT | bont/a2 | botA
Type:Metalloprotease
Mol. Mass.:48687.43
Organism:Clostridium botulinum
Description:Recombinant C. botulinum LC/A (1-425) was expressed in E. coli.
Residue:425
Sequence:
MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLN
PPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGG
STIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHDVLNLTRNGY
GSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAEHRLYGIAINPN
RVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDVASTLNKA
KSIIGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVNFFKV
INRKTYLNFDKAVFRINIVPDENYTIKDGFNLKGANLSTNFNGQNTEINSRNFTRLKNFT
GLFEF
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  Blast E-value cutoff:
BDBM23297
SNAP-25 substrate peptide
Name:SNAP-25 substrate peptide
Synonyms:n/a
Type:Peptide
Mol. Mass.:1978.24
Organism:n/a
Description:It is an N-terminal acetylated, C-terminal aminated, synthetic peptide identical in sequence to residues 187-203 of SNAP-25.
Residue:17
Sequence:
SNKTRIDEANQRATKML