Ki Summary BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
TargetATP-dependent RNA helicase RhlE
LigandBDBM13313
Substrate/Competitorbiotinylated lamin B peptide substrate
Meas. Tech.PFT IC50 Determination
pH7.5±n/a
Temperature310.15±n/a K
IC50 8.9±n/a nM
Citation Glenn, MP; Chang, SY; Horney, C; Rivas, K; Yokoyama, K; Pusateri, EE; Fletcher, S; Cummings, CG; Buckner, FS; Pendyala, PR; Chakrabarti, D; Sebti, SM; Gelb, M; Van Voorhis, WC; Hamilton, AD Structurally simple, potent, Plasmodium selective farnesyltransferase inhibitors that arrest the growth of malaria parasites. J Med Chem49:5710-27 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
ATP-dependent RNA helicase RhlE
Name:ATP-dependent RNA helicase RhlE
Synonyms:Protein Farnesyltransferase (PFT) | Protein prenyltransferase, alpha subunit
Type:Enzyme
Mol. Mass.:54781.31
Organism:Plasmodium falciparum (malaria parasite P. falciparum)
Description:Q5E175
Residue:500
Sequence:
MGFTSLGLSAPILKAVEEQGYSTPSPIQLQAIPAVIEGKDVMAAAQTGTGKTAGFTLPLL
ERLSNGPKRKFNQVRALVLTPTRELAAQVHESVEKYSKNLPLTSDVVFGGVKVNPQMQRL
RRGVDVLVATPGRLLDLANQNAIKFDQLEILVLDEADRMLDMGFIHDIKKILAKLPKNRQ
NLLFSATFSDEIRQLAKGLVKDPVEISVAKRNTTAETVEQSVYVMDKGRKPKVLTKLIKD
NDWKQVLVFSKTKHGANRLAKTLEEKGVSAAAIHGNKSQGARTKALANFKSGQVRVLVAT
DIAARGLDIEQLPQVINVDLPKVPEDYVHRIGRTGRAGATGKAISFVSEDEAKELFAIER
LIQKVLPRHVLEGFEPVNKVPESKLDTRPIKPKKPKKPKAPRVEHKDGQRSGENRNGNKQ
GAKQGQKPATKRTPTNNPSGKKEGTDSDKKKRPFSGKPKTKGTGENRGNGSNFGKSKSTP
KSDVKPRRQGPRPARKPKAN
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM13313
biotinylated lamin B peptide substrate
Name:biotinylated lamin B peptide substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1715.01
Organism:n/a
Description:A human lamin-B carboxy-terminus sequence peptide (biotin-YRASNRSCAIM) is 3H-farnesylated at the cysteine residue when processed by farnesyltransferase.
Residue:15
Sequence:
biotin-YRASNRSCAIM