Reaction Details |
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Target | RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
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Ligand | BDBM16939 |
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Substrate/Competitor | biotinylated Akt peptide substrate |
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Meas. Tech. | Akt Kinase Assay |
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IC50 | 3.3±n/a nM |
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Citation | Zhu, GD; Gandhi, VB; Gong, J; Luo, Y; Liu, X; Shi, Y; Guan, R; Magnone, SR; Klinghofer, V; Johnson, EF; Bouska, J; Shoemaker, A; Oleksijew, A; Jarvis, K; Park, C; Jong, RD; Oltersdorf, T; Li, Q; Rosenberg, SH; Giranda, VL Discovery and SAR of oxindole-pyridine-based protein kinase B/Akt inhibitors for treating cancers. Bioorg Med Chem Lett16:3424-9 (2006) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
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Name: | RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
Synonyms: | AKT1 | AKT1_HUMAN | C-AKT | PKB | Protein kinase B (Akt 1) | Protein kinase B alpha | RAC | RAC-PK-alpha |
Type: | Enzyme |
Mol. Mass.: | 39513.28 |
Organism: | Homo sapiens (Human) |
Description: | Recombinant His-tagged Akt1 (S378A, S381A, T450D,
S473D; 139-480) |
Residue: | 342 |
Sequence: | AKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTE
NRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIV
SALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAP
EVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKA
LLAGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYF
DEEFTAQMITIDPPDQDDSMECVDSERRPHFPQFDYSASGTA
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BDBM16939 |
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biotinylated Akt peptide substrate |
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Name: | biotinylated Akt peptide substrate |
Synonyms: | n/a |
Type: | Biotinylated Peptide |
Mol. Mass.: | 3178.04 |
Organism: | n/a |
Description: | Km=15 uM |
Residue: | 28 |
Sequence: | biotin-Ahx-EELSPFRGRSRSAPPNLWAAQR
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