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TargetATP-dependent RNA helicase RhlE
LigandBDBM13307
Substrate/Competitorbiotinylated lamin B peptide substrate
Meas. Tech.PfPFT IC50 Determination
pH7.5±n/a
Temperature310.15±n/a K
IC50 0.5±n/a nM
Citation Hast, MA; Fletcher, S; Cummings, CG; Pusateri, EE; Blaskovich, MA; Rivas, K; Gelb, MH; Van Voorhis, WC; Sebti, SM; Hamilton, AD; Beese, LS Structural basis for binding and selectivity of antimalarial and anticancer ethylenediamine inhibitors to protein farnesyltransferase. Chem Biol16:181-92 (2009) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
ATP-dependent RNA helicase RhlE
Name:ATP-dependent RNA helicase RhlE
Synonyms:Protein Farnesyltransferase (PFT) | Protein prenyltransferase, alpha subunit
Type:Enzyme
Mol. Mass.:54781.31
Organism:Plasmodium falciparum (malaria parasite P. falciparum)
Description:Q5E175
Residue:500
Sequence:
MGFTSLGLSAPILKAVEEQGYSTPSPIQLQAIPAVIEGKDVMAAAQTGTGKTAGFTLPLL
ERLSNGPKRKFNQVRALVLTPTRELAAQVHESVEKYSKNLPLTSDVVFGGVKVNPQMQRL
RRGVDVLVATPGRLLDLANQNAIKFDQLEILVLDEADRMLDMGFIHDIKKILAKLPKNRQ
NLLFSATFSDEIRQLAKGLVKDPVEISVAKRNTTAETVEQSVYVMDKGRKPKVLTKLIKD
NDWKQVLVFSKTKHGANRLAKTLEEKGVSAAAIHGNKSQGARTKALANFKSGQVRVLVAT
DIAARGLDIEQLPQVINVDLPKVPEDYVHRIGRTGRAGATGKAISFVSEDEAKELFAIER
LIQKVLPRHVLEGFEPVNKVPESKLDTRPIKPKKPKKPKAPRVEHKDGQRSGENRNGNKQ
GAKQGQKPATKRTPTNNPSGKKEGTDSDKKKRPFSGKPKTKGTGENRGNGSNFGKSKSTP
KSDVKPRRQGPRPARKPKAN
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BDBM13307
biotinylated lamin B peptide substrate
Name:biotinylated lamin B peptide substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1715.01
Organism:n/a
Description:A human lamin-B carboxy-terminus sequence peptide (biotin-YRASNRSCAIM) is 3H-farnesylated at the cysteine residue when processed by farnesyltransferase.
Residue:15
Sequence:
biotin-YRASNRSCAIM