| Reaction Details |
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 | Report a problem with these data |
| Target | Polyunsaturated fatty acid 5-lipoxygenase |
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| Ligand | BDBM50012187 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | ChEMBL_1353587 (CHEMBL3270668) |
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| IC50 | 3300±n/a nM |
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| Citation |  Pergola, C; Gaboriaud-Kolar, N; Jestädt, N; König, S; Kritsanida, M; Schaible, AM; Li, H; Garscha, U; Weinigel, C; Barz, D; Albring, KF; Huber, O; Skaltsounis, AL; Werz, O Indirubin core structure of glycogen synthase kinase-3 inhibitors as novel chemotype for intervention with 5-lipoxygenase. J Med Chem57:3715-23 (2014) [PubMed] Article |
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| More Info.: | Get all data from this article, Assay Method |
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| Polyunsaturated fatty acid 5-lipoxygenase |
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| Name: | Polyunsaturated fatty acid 5-lipoxygenase |
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| Synonyms: | 5-LO | 5-Lipo-oxygenase (5-LOX) | 5-Lipoxygenase (5-LO) | 5-Lipoxygenase (LOX) | 5-Lipoygenase | 5-lipoxygenase/FLAP | ALOX5 | Arachidonate 5-lipoxygenase | LOG5 | LOX5_HUMAN |
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| Type: | Enzyme |
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| Mol. Mass.: | 77972.74 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Recombinant protein was purified from E. coli lysate. After ammonium sulfate precipitation and subsequent steps, the supernatant (S100) was used for 5-LO activity assay.
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| Residue: | 674 |
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| Sequence: | MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDE
ELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLA
RDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVL
NYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNG
CNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDP
CTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDF
HVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECG
LFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWE
AIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYL
TVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCW
HLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPY
YYLSPDRIPNSVAI
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| BDBM50012187 |
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| n/a |
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| Name | BDBM50012187 |
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| Synonyms: | INDIRUBIN 2 |
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| Type | Small organic molecule |
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| Emp. Form. | C16H10BrN3O2 |
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| Mol. Mass. | 356.174 |
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| SMILES | O\N=C1\C(\Nc2ccccc\12)=C1\C(=O)Nc2ccc(Br)cc12 |
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| Structure | 
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