Reaction Details |
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Target | Dimer of Gag-Pol polyprotein [489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
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Ligand | BDBM577 |
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Substrate/Competitor | fluorescence peptide substrate |
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Meas. Tech. | Fluorometric Activity Assay |
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pH | 5.5±n/a |
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Temperature | 303.15±n/a K |
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Ki | 58±15 nM |
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Comments | Determined with the fluorometric activity assay. |
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Citation | Hanlon, MH; Porter, DJ; Furfine, ES; Spaltenstein, A; Carter, HL; Danger, D; Shu, AY; Kaldor, IW; Miller, JF; Samano, VA Inhibition of wild-type and mutant human immunodeficiency virus type 1 proteases by GW0385 and other arylsulfonamides. Biochemistry43:14500-7 (2004) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Dimer of Gag-Pol polyprotein [489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
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Name: | Dimer of Gag-Pol polyprotein [489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
Synonyms: | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I, ..) |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | n/a |
Components: | This complex has 2 components. |
Component 1 |
Name: | Gag-Pol polyprotein [489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
Synonyms: | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I, ..) Chain A | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I, ..) Chain B | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I,S37N,M46I,I50V,I54V,I62V, L63P,A71V,V82A,L90M) | POL_HV1H2 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10760.98 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P04585[489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
Residue: | 99 |
Sequence: | PQVTLWQRPIVTIKIGGQQREALLDTGADDTVLEDINLPGRWKPKIIGGVGGFVKVRQYD
QVPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNF
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Component 2 |
Name: | Gag-Pol polyprotein [489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
Synonyms: | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I, ..) Chain A | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I, ..) Chain B | HIV-1 Protease Mutant (L10I,L19Q,K20R,E35D,M36I,S37N,M46I,I50V,I54V,I62V, L63P,A71V,V82A,L90M) | POL_HV1H2 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10760.98 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P04585[489-587,L499I,L508Q,K509R,E524D,M525I,S526N,M535I,I539V,I543V,I551V,L552P,A560V,V571A,L579M] |
Residue: | 99 |
Sequence: | PQVTLWQRPIVTIKIGGQQREALLDTGADDTVLEDINLPGRWKPKIIGGVGGFVKVRQYD
QVPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNF
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BDBM577 |
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fluorescence peptide substrate |
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Name: | fluorescence peptide substrate |
Synonyms: | n/a |
Type: | Peptide |
Mol. Mass.: | 3467.28 |
Organism: | n/a |
Description: | n/a |
Residue: | 30 |
Sequence: | 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-NO2)-Gln-Arg-NH2
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