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TargetCyclin-dependent kinase 4/G1/S-specific cyclin-D1 [L188C]
LigandBDBM6643
Substrate/CompetitorG1 peptide
Meas. Tech.CDKs Assay
pH7.4±n/a
Temperature303.15±n/a K
IC50 36000±n/a nM
Citation Honma, T; Hayashi, K; Aoyama, T; Hashimoto, N; Machida, T; Fukasawa, K; Iwama, T; Ikeura, C; Ikuta, M; Suzuki-Takahashi, I; Iwasawa, Y; Hayama, T; Nishimura, S; Morishima, H Structure-based generation of a new class of potent Cdk4 inhibitors: new de novo design strategy and library design. J Med Chem44:4615-27 (2001) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Cyclin-dependent kinase 4/G1/S-specific cyclin-D1 [L188C]
Name:Cyclin-dependent kinase 4/G1/S-specific cyclin-D1 [L188C]
Synonyms:CDK4/Cyclin D | Cyclin-Dependent Kinase 4 (CDK4)
Type:Protein Complex
Mol. Mass.:n/a
Description:CDK4/D complexes were purified from insect cells co-infected with baculovirus vectors containing each of the components.
Components:This complex has 2 components.
Component 1
Name:Cyclin-dependent kinase 4
Synonyms:CDK4 | CDK4_HUMAN | Cell division protein kinase 4 | Cyclin-dependent kinase 4 (CDK 4) | PSK-J3
Type:Enzyme Subunit
Mol. Mass.:33731.96
Organism:Homo sapiens (Human)
Description:P11802
Residue:303
Sequence:
MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALL
RRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDL
MRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWY
RAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPR
DVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEG
NPE
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Component 2
Name:G1/S-specific cyclin-D1 [L188C]
Synonyms:BCL1 | CCND1 | CCND1_HUMAN | Cyclin D | PRAD1
Type:Enzyme Subunit
Mol. Mass.:33634.57
Organism:Homo sapiens (Human)
Description:P24385[L188C]
Residue:295
Sequence:
MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIV
ATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLT
AEKLCIYTDGSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRK
HAQTFVASCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCD
PDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
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BDBM6643
G1 peptide
Name:G1 peptide
Synonyms:n/a
Type:Peptide
Mol. Mass.:1483.28
Organism:n/a
Description:n/a
Residue:13
Sequence:
RPPTLSPIPHIPR