new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Enzyme Inhibition Measurements
Description:  Enzyme activities were assayed by monitoring the hydrolysis of substrate in the presence or absence of inhibitor compounds. The hydrolysis was recorded as the increase in fluorescence intensity over a 10 min time period. IC50 values were obtained by assuming competitive inhibition and fitting the dose response data to the equation (Vi/V0) =1/ (1 + [I]/IC50)). The Ki was calculated by using Ki = IC50/ (1 + [S]/Km), and a Km value determined according to Michaelis-Menten.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.