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Assay Method Information

Assay Name:  BoNT/A LC Metalloprotease Activity Assay
Description:  Botox A catalyzed the hydrolysis of substrate peptide between residues 11 (glutamine) and 12 (arginine), corresponding to residues 197 and 198 of SNAP-25. Percent inhibition measurements were performed in duplicate, and in all cases standard deviations were less than +/-25%. IC50 values for the inhibitors were calculated from plots of concentration versus inhibition using 7-9 concentrations of inhibitors.
Affinity data for this assay
 

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Last update November 1, 2007
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