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Assay Method Information |
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| | Amidolytic Activity Assay (Urokinase) |
| Description: | Determination of amidolytic activity was performed as described previously [Okada et al., Chem. Pharm. Bull., 36:1289-1297]. Detailed description of the method is given below. To 0.2 cm3 of examined compound (1−11; 0.15 M NaCl as control), buffer, and 0.1 cm^3 of enzyme solution was added: tris buffer−0.6 cm3 (pH = 8.8); enzyme: urokinase (50 units/cm^3); synthetic substrate: S-2444 (0.1 cm^3, 3 mM/dm^3). |
| Affinity data for this assay | |
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