Found 6 Enz. Inhib. hit(s) with Target = 'Prostaglandin E2 receptor EP4 subtype' and Ligand = 'BDBM190270' TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataKi: 0.200nMAssay Description:Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cell membranes after 120 mins by liquid scintillation counting metho...More data for this Ligand-Target Pair
TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataKi: 0.210nM IC50: 0.570nMpH: 6.0Assay Description:Assay Volume and Format:200 μl in 96-well plateCell membrane homogenates (20 μg protein) are incubated for 120 min at 22° C. with 0.5 nM [3...More data for this Ligand-Target Pair
TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataEC50: 0.348nMAssay Description:Agonist activity at human EP4 receptor expressed in HEK293T/17 cells assessed as increase in intracellular cAMP level incubated for 30 mins by ELISAMore data for this Ligand-Target Pair
TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataEC50: 0.0210nMAssay Description:Agonist activity at human EP4 receptor expressed in HEK293T/17 cells assessed as increase in GalphaS-mediated CREB activation measured after 6 to 24 ...More data for this Ligand-Target Pair
TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataEC50: 0.370nMT: 2°CAssay Description:1. Seed cells on an EP2 or EP4 STEP plate at a density of 40,000-80,000 cells/well in 200 uL of reduced serum medium containing 0.5% FBS. Place the p...More data for this Ligand-Target Pair
TargetProstaglandin E2 receptor EP4 subtype(Homo sapiens (Human))
Cayman Chemical Company, Inc.
Curated by ChEMBL
Cayman Chemical Company, Inc.
Curated by ChEMBL
Affinity DataEC50: 0.0590nMT: 2°CAssay Description:1. Seed cells on an EP2 or EP4 STEP plate at a density of 40,000-80,000 cells/well in 200 ul of reduced serum medium containing 0.5% FBS. Place the p...More data for this Ligand-Target Pair
