Affinity DataIC50: 600nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 1.00E+4nMAssay Description:Inhibition of human GST-tagged UCHL3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine1...More data for this Ligand-Target Pair
Affinity DataIC50: 1.41E+4nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 1.54E+4nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 1.70E+4nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 2.30E+4nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 2.48E+4nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 2.50E+4nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 3.21E+4nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 4.70E+4nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 5.26E+4nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 7.10E+4nMAssay Description:Inhibition of UCHL3 in human H1299 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 7.40E+4nMAssay Description:Inhibition of recombinant human UCHL3 assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using...More data for this Ligand-Target Pair
Affinity DataIC50: 8.00E+4nMAssay Description:Inhibition of human GST-tagged UCHL3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine1...More data for this Ligand-Target Pair
Affinity DataIC50: 8.00E+4nMAssay Description:Inhibition of human GST-tagged UCHL3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine1...More data for this Ligand-Target Pair
Affinity DataIC50: 1.03E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 1.14E+5nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 1.14E+5nMpH: 7.5 T: 2°CAssay Description:Ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) was generated as previously described.(7) The enzymatic reaction was conducted in fluorometric assay buff...More data for this Ligand-Target Pair
Affinity DataIC50: 1.23E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 1.54E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 2.00E+5nMAssay Description:Inhibition of UCH-L3 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by flu...More data for this Ligand-Target Pair
Ligand Info
In DepthDetails
Affinity DataIC50: 3.31E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 3.34E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 3.86E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 3.87E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair
Affinity DataIC50: 4.01E+5nMpH: 7.8 T: 2°CAssay Description:Enzyme reactions were carried out on Costar 96-well black assay plates. After the reaction, AMC fluorescence (excitation wavelength: 355 nm, emission...More data for this Ligand-Target Pair