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TargetDNA polymerase alpha subunit B
LigandBDBM33385
Substrate/CompetitorDNA template-primer
Meas. Tech.DNA Polymerase Assay
pH7.5±n/a
Temperature310.15±n/a K
IC50 890±n/a nM
Citation Kuramochi, K; Fukudome, K; Kuriyama, I; Takeuchi, T; Sato, Y; Kamisuki, S; Tsubaki, K; Sugawara, F; Yoshida, H; Mizushina, Y Synthesis and structure-activity relationships of dehydroaltenusin derivatives as selective DNA polymerase alpha inhibitors. Bioorg Med Chem17:7227-38 (2009) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
DNA polymerase alpha subunit B
Name:DNA polymerase alpha subunit B
Synonyms:70 kD subunit | DNA Polymerase alpha | DNA polymerase alpha 70 kDa subunit | DNA polymerase alpha subunit B | DPOA2_BOVIN | POLA2 | Polymerase (DNA directed), alpha 2
Type:Enzyme Subunit
Mol. Mass.:66940.74
Organism:Bos taurus (bovine)
Description:Enzyme was purchased from Ulrich Hubscher, University of Zurich-Irchel, Switzerland.
Residue:604
Sequence:
MAVSAQLLVEELQIFGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLE
TLNSFEHEFLSKRVSKTRHGASKDKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSK
GSQKRTITTPETPLTKRSVSARSPHQLLSPSSFSPSATPPQKYSSRSNRGEVVTSFGSAQ
GVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKEHYKIE
AFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKEYSLFP
GQVVVMEGINTTGRKLVATRLYEGVPLPFHQPDEEDGDSEQFMVLVACGPYTTSDSITFD
PLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTSSFEDVFKQCLRTIIEGTRSSGS
HLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRVRLVSEPCTLSINGVIFGLTSTDLLF
HMGAEEISSSSGTSDRFSRILRHILTQRSYYPLYPPQEDMAIDYENFYLYAQLPVTPDVF
IAPSELRYFVKVGLNSAISLQCQNQFLSGALALNSSRVCWWGQWPRSFGGGVGVENKSVS
IMLK
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM33385
DNA template-primer
Name:DNA template-primer
Synonyms:poly(dA)/oligo(dT)(12Â?1), A/T = 2/1
Type:Duplex DNA
Mol. Mass.:358.43
Organism:n/a
Description:[3H]-dTTP as co-substrate.
Residue:3
Sequence:
n/a