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TargetCyclin-A2/Cyclin-dependent kinase 1
LigandBDBM17140
Substrate/Competitorbiotinylated CDK peptide substrate
Meas. Tech.CDK Activity Assay.
pH7.4±n/a
Temperature295.15±n/a K
Ki 23±6 nM
CommentsThe apparent Km for ATP in the CDK1 assay was about 31 uM.
Citation Zhao, B; Bower, MJ; McDevitt, PJ; Zhao, H; Davis, ST; Johanson, KO; Green, SM; Concha, NO; Zhou, BB Structural basis for Chk1 inhibition by UCN-01. J Biol Chem277:46609-15 (2002) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Cyclin-A2/Cyclin-dependent kinase 1
Name:Cyclin-A2/Cyclin-dependent kinase 1
Synonyms:CDK1/Cyclin A | Cyclin-Dependent Kinase 1 (CDK1)
Type:Protein Complex
Mol. Mass.:n/a
Description:CDK1 was expressed in a baculovirus expression system and was partially purified. Cyclin A was purified separately and mixed with CDK1 before assays.
Components:This complex has 2 components.
Component 1
Name:Cyclin-dependent kinase 1
Synonyms:CDC2 | CDC28A | CDK1 | CDK1_HUMAN | CDKN1 | Cell division control protein 2 homolog | Cell division protein kinase 1 | Cyclin-dependent kinase 1 (CDK1) | P34CDC2 | p34 protein kinase
Type:Enzyme Subunit
Mol. Mass.:34101.08
Organism:Homo sapiens (Human)
Description:P06493
Residue:297
Sequence:
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRH
PNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCH
SRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSAR
YSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNT
FPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
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Component 2
Name:Cyclin-A2
Synonyms:CCN1 | CCNA | CCNA1 | CCNA2 | CCNA2_HUMAN | Cyclin A | Cyclin-A
Type:Enzyme Subunit
Mol. Mass.:48550.19
Organism:Homo sapiens (Human)
Description:P20248
Residue:432
Sequence:
MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRG
LAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKI
EREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHE
DIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYID
RFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLK
VLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAF
HLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHG
VSLLNPPETLNL
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BDBM17140
biotinylated CDK peptide substrate
Name:biotinylated CDK peptide substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:2939.48
Organism:n/a
Description:n/a
Residue:25
Sequence:
Biotin-aminohexyl-ARRPMSPKKKA-NH2