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TargetE3 ubiquitin-protein ligase XIAP [241-356]
LigandBDBM13211
Substrate/CompetitorSmac-derived peptide
Meas. Tech.Fluorescence Polarization Assay
pH7.5±n/a
Temperature295.15±n/a K
Ki 420±n/a nM
IC50 460±30 nM
Citation Mastrangelo, E; Cossu, F; Milani, M; Sorrentino, G; Lecis, D; Delia, D; Manzoni, L; Drago, C; Seneci, P; Scolastico, C; Rizzo, V; Bolognesi, M Targeting the X-linked inhibitor of apoptosis protein through 4-substituted azabicyclo[5.3.0]alkane smac mimetics. Structure, activity, and recognition principles. J Mol Biol384:673-89 (2008) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
E3 ubiquitin-protein ligase XIAP [241-356]
Name:E3 ubiquitin-protein ligase XIAP [241-356]
Synonyms:API3 | BIR3 domain of X-linked inhibitor of apoptosis protein | BIRC4 | E3 ubiquitin-protein ligase (XIAP-BIR3) | E3 ubiquitin-protein ligase XIAP BIR-3 | E3 ubiquitin-protein ligase XIAP BIR3 | IAP3 | X chromosome-linked inhibitor of apoptosis BIR3 domain (XIAP BIR3) | X-linked inhibitor of apoptosis protein BIR3 domain (XIAP BIR-3) | XIAP | XIAP-BIR3 | XIAP_HUMAN | baculovirus IAP repeat 3 (BIR3) domain of XIAP
Type:Protein Binding Domain
Mol. Mass.:13276.62
Organism:Homo sapiens (Human)
Description:BIR3 of XIAP: RESIDUES 241-356.
Residue:116
Sequence:
SDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKC
FHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTT
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM13211
Smac-derived peptide
Name:Smac-derived peptide
Synonyms:SM5F
Type:fluorescently labelled peptide
Mol. Mass.:1220.95
Organism:n/a
Description:5-carboxyfluorescein was coupled to the lysine side chain of the mutated Smac peptide.
Residue:10
Sequence:
AbuRPF-K(5-Fam)-NH2