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TargetMAP kinase-activated protein kinase 2 [41-364]
LigandBDBM30266
Substrate/CompetitorBiotinylated GST-LSP1
Meas. Tech.Dissociation-Enhanced Lanthanide Fluorescent Immunoassay (DELFIA)
IC50 35±n/a nM
Citation Wu, JP; Wang, J; Abeywardane, A; Andersen, D; Emmanuel, M; Gautschi, E; Goldberg, DR; Kashem, MA; Lukas, S; Mao, W; Martin, L; Morwick, T; Moss, N; Pargellis, C; Patel, UR; Patnaude, L; Peet, GW; Skow, D; Snow, RJ; Ward, Y; Werneburg, B; White, A The discovery of carboline analogs as potent MAPKAP-K2 inhibitors. Bioorg Med Chem Lett17:4664-9 (2007) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
MAP kinase-activated protein kinase 2 [41-364]
Name:MAP kinase-activated protein kinase 2 [41-364]
Synonyms:MAPK-Activated Protein Kinase 2 (MK2) | MAPK2_HUMAN | MAPKAP kinase 2 | MAPKAPK-2 | MAPKAPK2
Type:Serine/threonine-protein kinase
Mol. Mass.:37477.48
Organism:Homo sapiens (Human)
Description:MK2 was activated with p38alpha MAPK and re-purified by GSH affinity chromatography.
Residue:324
Sequence:
QQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPK
ARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTE
REASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSL
TTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIR
MGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSR
VLKEDKERWEDVKEEMTSALATMR
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  Blast E-value cutoff:
BDBM30266
Biotinylated GST-LSP1
Name:Biotinylated GST-LSP1
Synonyms:lymphocyte-specific protein 1
Type:Other Protein Type
Mol. Mass.:17410.08
Organism:n/a
Description:The GST-LSP1 179-339 substrate of MK2 was biotinylated with a 70-fold molar excess of PEO-iodoacetyl biotin (Pierce), followed by quenching with excess DTT and purification by Sephadex G-25 gel filtration.
Residue:161
Sequence:
LVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAI
ETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWE
QKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP