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TargetE3 ubiquitin-protein ligase Mdm2
LigandBDBM31285
Substrate/CompetitorFl 9mer
Meas. Tech.Fluorescent Peptide Displacement Assay
pH7.5±n/a
Temperature296.15±n/a K
IC50 420±n/a nM
Citation Parks, DJ; LaFrance, LV; Calvo, RR; Milkiewicz, KL; Marugán, JJ; Raboisson, P; Schubert, C; Koblish, HK; Zhao, S; Franks, CF; Lattanze, J; Carver, TE; Cummings, MD; Maguire, D; Grasberger, BL; Maroney, AC; Lu, T Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design. Bioorg Med Chem Lett16:3310-4 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
E3 ubiquitin-protein ligase Mdm2
Name:E3 ubiquitin-protein ligase Mdm2
Synonyms:Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:Oncoprotein
Mol. Mass.:55196.54
Organism:Homo sapiens (Human)
Description:Q00987
Residue:491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQY
IMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGT
SVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQ
RKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS
VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLA
DYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVP
DCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQ
DKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQ
PIQMIVLTYFP
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM31285
Fl 9mer
Name:Fl 9mer
Synonyms:n/a
Type:fluorescent peptide
Mol. Mass.:2263.48
Organism:n/a
Description:n/a
Residue:18
Sequence:
fluorescein-FMDYWEGLN