Ki Summary

Reaction Details

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Target

Biotin carboxylase

Ligand

BDBM32644

Substrate/Competitor

Biotinyl-BCCP

Meas. Tech.

ACCase-Coupled Enzyme Assay

8±n/a

Temperature

296.15±n/a K

IC50

333±n/a nM

Citation

Mochalkin, I; Miller, JR; Narasimhan, L; Thanabal, V; Erdman, P; Cox, PB; Prasad, JV; Lightle, S; Huband, MD; Stover, CK Discovery of antibacterial biotin carboxylase inhibitors by virtual screening and fragment-based approaches. ACS Chem Biol4:473-83 (2009) [PubMed] Article

More Info.:

Get all data from this article, Solution Info, Assay Method

Biotin carboxylase

Name:

Biotin carboxylase

Synonyms:

ACC | ACCC_ECOLI | Acetyl-CoA carboxylase subunit A | accC | fabG

Type:

A component of the acetyl coenzyme A carboxylase complex

Mol. Mass.:

49324.23

Organism:

Escherichia coli (strain K12)

Description:

The accC gene was amplified by PCR from E. coli genomic DNA. The recombinant protein was expressed and purified from E. coli.

Residue:

449

Sequence:

MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLLADETVCIGPAPSVKSY
LNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIFIGPKAETIRLMGDKVSAI
AAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKASGGGGGRGMRVVRGDAELAQ
SISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADGQGNAIYLAERDCSMQRRHQKVV
EEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFEFLFENGEFYFIEMNTRIQVEHPVT
EMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHAVECRINAEDPNTFLPSPGKITRFHAP
GGFGVRWESHIYAGYTVPPYYDSMIGKLICYGENRDVAIARMKNALQELIIDGIKTNVDL
QIRIMNDENFQHGGTNIHYLEKKLGLQEK

BDBM32644

Biotinyl-BCCP

Name:

Biotinyl-BCCP

Synonyms:

BCCP_ECOLI | Biotin carboxyl carrier protein of acetyl-CoA carboxylase | accB | fabE

Type:

biotin binding protein

Mol. Mass.:

16678.47

Organism:

Escherichia coli (strain K12)

Description:

n/a

Residue:

156

Sequence:

MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVMQQAYAAPMMQQPAQSN
AAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAFIEVGQKVNVGDTLCIVEA
MKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE