Reaction Details |
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Target | Genome polyprotein [1066-1250,R1182K]/[1394-1440,K1405R,D1408E] |
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Ligand | BDBM33256 |
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Substrate/Competitor | BDBM33248 |
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Meas. Tech. | Enzyme Inhibition Assay (IC50) and Fluorescence Quench Assay (KD) |
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pH | 7.5±n/a |
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Temperature | 310.15±n/a K |
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IC50 | 1600±800 nM |
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Comments | Hill slope=3.2. |
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Citation | Bodenreider, C; Beer, D; Keller, TH; Sonntag, S; Wen, D; Yap, L; Yau, YH; Shochat, SG; Huang, D; Zhou, T; Caflisch, A; Su, XC; Ozawa, K; Otting, G; Vasudevan, SG; Lescar, J; Lim, SP A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease. Anal Biochem395:195-204 (2009) [PubMed] Article |
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More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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Genome polyprotein [1066-1250,R1182K]/[1394-1440,K1405R,D1408E] |
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Name: | Genome polyprotein [1066-1250,R1182K]/[1394-1440,K1405R,D1408E] |
Synonyms: | Dengue 2 NS2B(H):NS3 | Dengue Virus 2 (DENV2) NS2B-NS3 Protease |
Type: | Cofactor/Enzyme |
Mol. Mass.: | n/a |
Description: | n/a |
Components: | This complex has 2 components. |
Component 1 |
Name: | Genome polyprotein [1394-1440,K1405R,D1408E] |
Synonyms: | Flavivirus non-structural protein NS2B | NS2B | POLG_DEN2N |
Type: | Cofactor Domain |
Mol. Mass.: | 5197.48 |
Organism: | Dengue virus 2 (strain TSV01) |
Description: | P14340[1394-1440,K1405R,D1408E] |
Residue: | 47 |
Sequence: | adleler aadvrweeqa eisgsspils itisedgsms ikneeeeqtl
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Component 2 |
Name: | Genome polyprotein [1066-1250,R1182K] |
Synonyms: | Flavivirus NS3 serine protease | NS3 | POLG_DEN2T |
Type: | Catalytic Domain |
Mol. Mass.: | 19925.90 |
Organism: | Dengue virus 2 (strain TSV01) |
Description: | n/a |
Residue: | 185 |
Sequence: | AGVLWDVPSPPPVGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMH
KGKRIEPSWADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNT
GTIGAVSLDFSPGTSGSPIVDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIED
DIFRK
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BDBM33256 |
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BDBM33248 |
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Name | BDBM33256 |
Synonyms: | phthalazine-based compound, 7 |
Type | Small organic molecule |
Emp. Form. | C31H26N6O2 |
Mol. Mass. | 514.5771 |
SMILES | COC(=O)c1ccc(cc1)-c1ccc(Nc2nnc(Nc3ccc(cc3)C3=NCCN3)c3ccccc23)cc1 |t:28| |
Structure |
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