| Reaction Details |
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 | Report a problem with these data |
| Target | Genome polyprotein [1424-1463]/[1502-1685] |
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| Ligand | BDBM33255 |
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| Substrate/Competitor | BDBM33248 |
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| Meas. Tech. | Enzyme Inhibition Assay (IC50) and Fluorescence Quench Assay (KD) |
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| pH | 7.5±n/a |
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| Temperature | 310.15±n/a K |
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| IC50 | 6200±n/a nM |
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| Kd | 7000±n/a nM |
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| Comments | Hill slope=1.1. |
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| Citation |  Bodenreider, C; Beer, D; Keller, TH; Sonntag, S; Wen, D; Yap, L; Yau, YH; Shochat, SG; Huang, D; Zhou, T; Caflisch, A; Su, XC; Ozawa, K; Otting, G; Vasudevan, SG; Lescar, J; Lim, SP A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease. Anal Biochem395:195-204 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Genome polyprotein [1424-1463]/[1502-1685] |
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| Name: | Genome polyprotein [1424-1463]/[1502-1685] |
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| Synonyms: | WNV CF40.Gly.NS3Pro | WNV NS2B(H):NS3 | West Nile Virus (WNV) NS2B-NS3 Protease |
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| Type: | Cofactor/Enzyme |
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| Mol. Mass.: | n/a |
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| Description: | It is the catalytically active recombinant WNV protease, consisting of a 40-residue component of cofactor NS2B tethered via a nonapeptide (G4SG4) to the N-terminal 184 residues of NS3. |
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| Components: | This complex has 2 components. |
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| Component 1 |
| Name: | Genome polyprotein [1424-1463] |
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| Synonyms: | Flavivirin protease NS2B regulatory subunit | NS2B | POLG_WNV |
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| Type: | Cofactor Domain |
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| Mol. Mass.: | 4431.59 |
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| Organism: | West Nile virus (WNV) |
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| Description: | P06935[1424-1463] |
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| Residue: | 40 |
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| Sequence: | IERTADITWESDAEITGSSERVDVRLDDDGNFQLMNDPGA
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| Component 2 |
| Name: | Genome polyprotein [1502-1685] |
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| Synonyms: | Flavivirin protease NS3 catalytic subunit | NS3 | POLG_WNV |
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| Type: | Catalytic Subunit |
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| Mol. Mass.: | 19839.27 |
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| Organism: | West Nile virus (WNV) |
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| Description: | P06935[1502-1685] |
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| Residue: | 184 |
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| Sequence: | GGVLWDTPSPKEYKKGDTTTGVYRIMTRGLLGSYQAGAGVMVEGVFHTLWHTTKGAALMS
GEGRLDPYWGSVKEDRLCYGGPWKLQHKWNGHDEVQMIVVEPGKNVKNVQTKPGVFKTPE
GEIGAVTLDYPTGTSGSPIVDKNGDVIGLYGNGVIMPNGSYISAIVQGERMEEPAPAGFE
PEML
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| BDBM33255 |
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| BDBM33248 |
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| Name | BDBM33255 |
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| Synonyms: | phthalazine-based compound, 6 |
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| Type | Small organic molecule |
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| Emp. Form. | C26H25N7 |
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| Mol. Mass. | 435.5236 |
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| SMILES | C1CN=C(N1)c1ccc(Nc2nnc(Nc3ccc4CNCCc4c3)c3ccccc23)cc1 |c:2| |
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| Structure | 
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