Reaction Details |
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Target | Reverse transcriptase/RNaseH |
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Ligand | BDBM33410 |
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Substrate/Competitor | RNA/DNA hybrid |
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Meas. Tech. | Fluorescence-Based RNase H Assay |
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pH | 8±n/a |
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Temperature | 293.15±n/a K |
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IC50 | 135±n/a nM |
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Comments | IC50 is the mean of 0.12 and 0.15 uM. |
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Citation | Kirschberg, TA; Balakrishnan, M; Squires, NH; Barnes, T; Brendza, KM; Chen, X; Eisenberg, EJ; Jin, W; Kutty, N; Leavitt, S; Liclican, A; Liu, Q; Liu, X; Mak, J; Perry, JK; Wang, M; Watkins, WJ; Lansdon, EB RNase H active site inhibitors of human immunodeficiency virus type 1 reverse transcriptase: design, biochemical activity, and structural information. J Med Chem52:5781-4 (2009) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Reverse transcriptase/RNaseH |
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Name: | Reverse transcriptase/RNaseH |
Synonyms: | HIV-1 Reverse Transcriptase RNase H | Human immunodeficiency virus type 1 reverse transcriptase | Reverse transcriptase/RNaseH |
Type: | PROTEIN |
Mol. Mass.: | 65229.15 |
Organism: | Human immunodeficiency virus 1 |
Description: | ChEMBL_1473730 |
Residue: | 566 |
Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKRKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIRVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLRTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVLFLDGID
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BDBM33410 |
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RNA/DNA hybrid |
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Name: | RNA/DNA hybrid |
Synonyms: | n/a |
Type: | fluorescein-labeled RNA annealed to DNA |
Mol. Mass.: | 2134.40 |
Organism: | n/a |
Description: | It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA. |
Residue: | 23 |
Sequence: | 5-GAU CUG AGC CUG GGA GCU-fluorescein-3
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