| Reaction Details |
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 | Report a problem with these data |
| Target | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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| Ligand | BDBM5485 |
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| Substrate/Competitor | Histone H1 |
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| Meas. Tech. | Kinase Inhibition Assay |
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| pH | 7.5±n/a |
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| Temperature | 303.15±n/a K |
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| Ki | 5000±1000 nM |
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| Comments | To obtain Ki value, the assay was performed in the absence of inhibitor and at two fixed inhibitor concentrations, 25 and 50 uM, with ATP concentrations ranging from 6.25 to 800 uM. Data were fitted to the Michaelis-Menten equation using unweighted nonlinear least squares regression. |
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| Citation |  Arris, CE; Boyle, FT; Calvert, AH; Curtin, NJ; Endicott, JA; Garman, EF; Gibson, AE; Golding, BT; Grant, S; Griffin, RJ; Jewsbury, P; Johnson, LN; Lawrie, AM; Newell, DR; Noble, ME; Sausville, EA; Schultz, R; Yu, W Identification of novel purine and pyrimidine cyclin-dependent kinase inhibitors with distinct molecular interactions and tumor cell growth inhibition profiles. J Med Chem43:2797-804 (2000) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| |
| Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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| Name: | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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| Synonyms: | CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B |
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| Type: | Protein Complex |
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| Mol. Mass.: | n/a |
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| Description: | p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography. |
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| Components: | This complex has 2 components. |
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| Component 1 |
| Name: | Cyclin-dependent kinase 1 |
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| Synonyms: | CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1 |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 34693.35 |
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| Organism: | Marthasterias glacialis (starfish) |
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| Description: | Q9DGD3 {empty hard to find) |
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| Residue: | 303 |
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| Sequence: | MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKH
PNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCH
RRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPR
YSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNT
FPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACI
NGV
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| Component 2 |
| Name: | G2/mitotic-specific cyclin-B |
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| Synonyms: | CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1 |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 43790.28 |
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| Organism: | Marthasterias glacialis (starfish) |
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| Description: | n/a |
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| Residue: | 388 |
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| Sequence: | MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGM
TKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFD
NPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQET
LFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQI
RSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPS
EIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKK
YSSAKFMNVSTISALTSSTVMDLADQMC
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| BDBM5485 |
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| Histone H1 |
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| Name: | Histone H1 |
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| Synonyms: | Sigma Type IIIS |
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| Type: | Other Protein Type |
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| Mol. Mass.: | 358.43 |
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| Organism: | Bos taurus (bovine) |
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| Description: | 12.5 uM ATP as co-substrate. |
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| Residue: | 3 |
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| Sequence: | |
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