| Reaction Details |
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 | Report a problem with these data |
| Target | Polyunsaturated fatty acid 5-lipoxygenase |
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| Ligand | BDBM22326 |
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| Substrate/Competitor | BDBM22319 |
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| Meas. Tech. | Enzyme Inhibition Assay |
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| pH | 7.4±n/a |
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| Temperature | 310.15±n/a K |
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| IC50 | 3000±n/a nM |
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| Comments | 72 +/- 4 inhibition @ 10 uM. |
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| Citation |  Landwehr, J; George, S; Karg, EM; Poeckel, D; Steinhilber, D; Troschuetz, R; Werz, O Design and synthesis of novel 2-amino-5-hydroxyindole derivatives that inhibit human 5-lipoxygenase. J Med Chem49:4327-32 (2006) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Polyunsaturated fatty acid 5-lipoxygenase |
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| Name: | Polyunsaturated fatty acid 5-lipoxygenase |
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| Synonyms: | 5-LO | 5-Lipo-oxygenase (5-LOX) | 5-Lipoxygenase (5-LO) | 5-Lipoxygenase (LOX) | 5-Lipoygenase | 5-lipoxygenase/FLAP | ALOX5 | Arachidonate 5-lipoxygenase | LOG5 | LOX5_HUMAN |
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| Type: | Enzyme |
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| Mol. Mass.: | 77972.74 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Recombinant protein was purified from E. coli lysate. After ammonium sulfate precipitation and subsequent steps, the supernatant (S100) was used for 5-LO activity assay.
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| Residue: | 674 |
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| Sequence: | MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDE
ELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLA
RDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVL
NYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNG
CNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDP
CTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDF
HVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECG
LFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWE
AIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYL
TVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCW
HLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPY
YYLSPDRIPNSVAI
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| BDBM22326 |
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| BDBM22319 |
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| Name | BDBM22326 |
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| Synonyms: | 2-amino-5-hydroxyindole, 3l | ethyl 5-hydroxy-2-[(1-phenylethyl)amino]-1H-indole-3-carboxylate |
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| Type | Small organic molecule |
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| Emp. Form. | C19H20N2O3 |
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| Mol. Mass. | 324.3737 |
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| SMILES | CCOC(=O)c1c(NC(C)c2ccccc2)[nH]c2ccc(O)cc12 |
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| Structure | 
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