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TargetRenin
LigandBDBM18012
Substrate/CompetitorGreen Flourescent Protein (tGFP)
Meas. Tech.In Vitro Renin IC50 Determinations (Tandem GFP FRET Assay)
pH7.4±n/a
Temperature295.15±n/a K
IC50 0.067±n/a nM
Citation Holsworth, DD; Jalaie, M; Belliotti, T; Cai, C; Collard, W; Ferreira, S; Powell, NA; Stier, M; Zhang, E; McConnell, P; Mochalkin, I; Ryan, MJ; Bryant, J; Li, T; Kasani, A; Subedi, R; Maiti, SN; Edmunds, JJ Discovery of 6-ethyl-2,4-diaminopyrimidine-based small molecule renin inhibitors. Bioorg Med Chem Lett17:3575-80 (2007) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Renin
Name:Renin
Synonyms:Angiotensinogenase | REN | RENI_HUMAN
Type:Enzyme
Mol. Mass.:45058.99
Organism:Homo sapiens (Human)
Description:n/a
Residue:406
Sequence:
MDGWRRMPRWGLLLLLWGSCTFGLPTDTTTFKRIFLKRMPSIRESLKERGVDMARLGPEW
SQPMKRLTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRL
YTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEM
PALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGG
QIVLGGSDPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISG
STSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKK
LCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM18012
Green Flourescent Protein (tGFP)
Name:Green Flourescent Protein (tGFP)
Synonyms:n/a
Type:Other Protein Type
Mol. Mass.:358.43
Organism:n/a
Description:The tGFP substrate contained a nine amino acid (Ile-His-Pro-Phe-His-Leu-Val-Ile-His) recognition sequence for human renin flanked by two GPF proteins (W1B and Topaz).
Residue:3
Sequence:
n/a