| Reaction Details |
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 | Report a problem with these data |
| Target | Glutamate--tRNA ligase |
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| Ligand | BDBM18120 |
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| Substrate/Competitor | BDBM17657 |
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| Meas. Tech. | Determination of the Inhibition Type and Constant (Ki) |
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| pH | 7.2±n/a |
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| Temperature | 310.15±n/a K |
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| Ki | 2900000±n/a nM |
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| Km | 113000±n/a nM |
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| Comments | Competitive inhibition |
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| Citation |  Balg, C; Blais, SP; Bernier, S; Huot, JL; Couture, M; Lapointe, J; Chênevert, R Synthesis of beta-ketophosphonate analogs of glutamyl and glutaminyl adenylate, and selective inhibition of the corresponding bacterial aminoacyl-tRNA synthetases. Bioorg Med Chem15:295-304 (2007) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Glutamate--tRNA ligase |
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| Name: | Glutamate--tRNA ligase |
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| Synonyms: | Glutamate--tRNA ligase | Glutamyl-tRNA synthetase | SYE_ECOLI | gltX | tRNA synthetase (GluRS) |
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| Type: | Enzyme |
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| Mol. Mass.: | 53807.55 |
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| Organism: | Escherichia coli |
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| Description: | His6-tagged clone expressed in E. coli. |
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| Residue: | 471 |
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| Sequence: | MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
MNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPR
YDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPT
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNT
DKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQS
CRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITDWTAENVHHAIQATADELEVG
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALDFIAERENQQ
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| BDBM18120 |
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| BDBM17657 |
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| Name | BDBM18120 |
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| Synonyms: | Gln-KPA | [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl [(3S)-3-amino-5-carbamoyl-2-oxopentyl]phosphonate | beta-ketophosphonate analogue |
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| Type | Small organic molecule |
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| Emp. Form. | C16H23N7O8P |
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| Mol. Mass. | 472.3702 |
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| SMILES | N[C@@H](CCC(N)=O)C(=O)CP([O-])(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12 |r| |
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| Structure | 
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