| Reaction Details |
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 | Report a problem with these data |
| Target | DNA-directed RNA polymerase subunit beta [D516V] |
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| Ligand | BDBM228835 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | RNAP-Inhibition Assay |
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| Temperature | 310.15±n/a K |
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| IC50 | 4e+3±n/a nM |
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| Comments | extracted |
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| Citation |  Walker, SS; Degen, D; Nickbarg, E; Carr, D; Soriano, A; Mandal, M; Painter, RE; Sheth, P; Xiao, L; Sher, X; Murgolo, N; Su, J; Olsen, DB; Ebright, RH; Young, K Affinity Selection-Mass Spectrometry Identifies a Novel Antibacterial RNA Polymerase Inhibitor. ACS Chem Biol12:1346-1352 (2017) [PubMed] Article |
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| More Info.: | Get all data from this article, Assay Method |
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| |
| DNA-directed RNA polymerase subunit beta [D516V] |
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| Name: | DNA-directed RNA polymerase subunit beta [D516V] |
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| Synonyms: | RNA polymerase subunit beta ([D516V]β-RNAP) | RPOB_SHISS | rpoB |
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| Type: | Enzyme |
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| Mol. Mass.: | 150580.85 |
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| Organism: | Escherichia coli |
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| Description: | E. coli RNAP D516V mutant |
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| Residue: | 1342 |
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| Sequence: | MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMVQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
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| BDBM228835 |
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| n/a |
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| Name | BDBM228835 |
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| Synonyms: | MRL-436 |
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| Type | Small organic molecule |
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| Emp. Form. | C24H22N4O |
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| Mol. Mass. | 382.4577 |
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| SMILES | CC(C)Cc1nnc2c(cccn12)C(=O)NC1c2ccccc2-c2ccccc12 |
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| Structure | 
|
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